Cgas Knockout cell line (RAW264.7)

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location： Home > Products > KO Cell Lines > Cgas Knockout cell line (RAW264.7)

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Cgas Knockout cell line (RAW264.7) Order

Cgas Knockout cell line (RAW264.7)
Catalog#
YKO-H1065
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Cgas Knockout cell line (RAW264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Cgas Knockout cell line (RAW264.7)
Species
Mouse
Gene Name
Cgas
Gene ID
214763
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript Cgas-202)
Validation: PCR+Sanger Sequencing
Cgas Knockout cell line (RAW264.7) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Cgas gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the RAW264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Cgas gene knockout Cgas Knockout cell line (RAW264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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